牟川;陈庆果;

• 1:四川师范大学体育学院

摘要(Abstract)：

目的 探讨8周有氧运动对IR小鼠肝脏糖异生及FNDC5/Irisin/Akt/FoxO1/PEPCK/G6Pase信号的影响。方法 5周龄C57BL/6雄性小鼠随机分为对照组(Con组，n=16)和高脂膳食组(HFD组，n=34),干预12周建立IR模型。建模成功后，IR模型小鼠随机分为IR安静组(IR-Sed组，n=8)和IR运动组(IR-Ex组，n=8);Con组随机分为Con安静组(Con-Sed组，n=8)和Con运动组(Con-Ex组，n=8)。Con-Ex和IR-Ex组进行8周跑台运动(18 m/min, 60 min/天，5 d/周)。12周建模后和末次运动后检测胰岛素敏感性，末次运动48 h后取血和肝脏进行相关检测。结果 与Con组相比，HFD组体质量、FINS、FPG、HOMA-IR和AUC_(BG)均升高(P<0.01),ISI、葡萄糖耐量和胰岛素耐量均下降(P<0.01)。与IR-Sed组相比，8周有氧运动后IR-Ex组体质量、FINS、FPG、HOMA-IR和AUC_(BG)均降低(P<0.01),ISI、葡萄糖耐量和胰岛素耐量均升高(P<0.01)。同时，与Con-Sed组相比，IR-Sed组肝细胞发生脂肪变性，TG和血清ALT、AST含量均增加(P<0.01),PAS染色稀薄并呈不均匀样，肝糖原含量下降(P<0.01),PEPCK、G6Pase和FoxO1含量均增加(P<0.05),FNDC5、Irisin、p-Akt和p-FoxO1水平均降低(P<0.05);与IR-Sed组相比，8周有氧运动后IR-Ex组肝细胞脂肪变性得到改善，TG和血清ALT、AST含量均下降(P<0.01),PAS染色相对饱满均匀，肝糖原含量增加(P<0.05),PEPCK和G6Pase表达下降(P<0.01),FNDC5、Irisin、p-Akt和p-FoxO1水平均增加(P<0.01)。结论 长期有氧运动干预有效抑制肝脏糖异生并改善IR,可能与FNDC5/Irisin促进肝脏组织中Akt蛋白激活，进而促进FoxO1磷酸化使其蛋白失活，抑制糖异生关键酶PEPCK和G6Pase表达并改善肝脏功能有关。

关键词(KeyWords)： 有氧运动;胰岛素抵抗;糖异生;FNDC5/Irisin;肝脏

Abstract:

Keywords:

基金项目(Foundation): 国家体育总局重点科研项目(2014B039)

作者(Authors): 牟川;陈庆果;

DOI: 10.16063/j.cnki.issn1001-747x.2022.03.012

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